Characterization and quantitation of soybean proteins in commercial soybean products by capillary electrophoresis

Characterization and quantitation of soybean proteins in commercial soybean products by capillary electrophoresis.

Capillary electrophoresis was applied for the first time to determine soybean proteins in commercial soybean products. The most suitable conditions for the analysis of these products in less than 7 min were 0.05 M phosphate buffer (pH 8) with 1 M urea; detection wavelength, 254 nm; applied voltage, 20 kV; and temperature, 30 degrees C. Quantitation of soybean proteins was achieved using referen...

Analysis of bovine whey proteins in soybean dairy-like products by capillary electrophoresis.

The simultaneous separation of bovine whey proteins [alpha-lactalbumin and beta-lactoglobulin (A+B)] and soybean proteins was performed, for the first time, by capillary electrophoresis. Different experimental conditions were tested. The most suitable consisted of 0.050 M phosphate buffer (pH 8) with 1 M urea and 1.2 mg/ml methylhydroxyethylcellulose, UV detection at 280 nm, 15 kV applied volta...

Polyacrylamide-gel electrophoresis of soybean whey proteins and trypsin inhibitors.

Polyacrylamide·.g~1 electrophoresis with glycine buffer (pH 9.2) containing 8 M urca separated soybean whey proteins into at least 24 bands. In contrast, ultracentrifugation indicated only 2 fractions; moving-boundary electrophoresis, 8-9 components; and column chromatography, 13 or more proteins. A prominent, fast-moving band in the gel pattern appeared to be identical to crystalline soybean t...

High Pressure Treatments of Soybean and Soybean Products

Separation and identification of soybean leaf proteins by two-dimensional gel electrophoresis and mass spectrometry.

To establish a proteomic reference map for soybean leaves, we separated and identified leaf proteins using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry (MS). Tryptic digests of 260 spots were subjected to peptide mass fingerprinting (PMF) by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) MS. Fifty-three of these protein spots were id...